Evaluation and Optimization of Methods for Generating High-Resolution Retinotopic Maps Using Visual Cortex Voltage-Sensitive Dye Imaging

Carmi, Ori and Gross, Adi and Ivzan, Nadav and Franca, Lamberto La and Farah, Nairouz and Zalevsky, Zeev and Mandel, Yossi (2021) Evaluation and Optimization of Methods for Generating High-Resolution Retinotopic Maps Using Visual Cortex Voltage-Sensitive Dye Imaging. Frontiers in Cellular Neuroscience, 15. ISSN 1662-5102

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Abstract

The localization and measurement of neuronal activity magnitude at high spatial and temporal resolution are essential for mapping and better understanding neuronal systems and mechanisms. One such example is the generation of retinotopic maps, which correlates localized retinal stimulation with the corresponding specific visual cortex responses. Here we evaluated and compared seven different methods for extracting and localizing cortical responses from voltage-sensitive dye imaging recordings, elicited by visual stimuli projected directly on the rat retina by a customized projection system. The performance of these methods was evaluated both qualitatively and quantitatively by means of two cluster separation metrics, namely, the (adjusted) Silhouette Index (SI) and the (adjusted) Davies-Bouldin Index (DBI). These metrics were validated using simulated data, which showed that Temporally Structured Component Analysis (TSCA) outperformed all other analysis methods for localizing cortical responses and generating high-resolution retinotopic maps. The analysis methods, as well as the use of cluster separation metrics proposed here, can facilitate future research aiming to localize specific activity at high resolution in the visual cortex or other brain areas.

Item Type: Article
Subjects: Article Paper Librarian > Medical Science
Depositing User: Unnamed user with email support@article.paperlibrarian.com
Date Deposited: 12 Apr 2023 08:07
Last Modified: 02 May 2024 09:36
URI: http://editor.journal7sub.com/id/eprint/645

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