Improvement of Multiple-Locus VNTR Analysis Typing Scheme for Helicobacter pylori

Sorokin, Vladimir M. and Pisanov, Ruslan V. and Vodop'janov, Aleksej S. (2019) Improvement of Multiple-Locus VNTR Analysis Typing Scheme for Helicobacter pylori. Asian Journal of Biochemistry, Genetics and Molecular Biology, 1 (4). pp. 1-7. ISSN 2582-3698

[thumbnail of Sorokin142018AJBGMB46609.pdf]

Text
Sorokin142018AJBGMB46609.pdf - Published Version
Download (253kB)

Official URL: https://doi.org/10.9734/ajbgmb/2018/v1i430045

Abstract

Aims: To improve a multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) assay for Helicobacter pylori typing.

Materials and Methods: Polymorphic VNTRs were searched by Gene Expert. The distribution and polymorphism of each VNTR locus were analyzed in 18 H. pylori genomes from the NCBI genome database by BLAST and were compared with a collection of 15 clinical H. pylori strains. The MLVA assay was compared with MLST-typing for discriminating H. pylori isolates.

Results: Twelve VNTR loci were identified by bioinformatic screening of H. pylori genomes, and five of them were highly polymorphic. Therefore, an MLVA assay composed of five VNTR loci was developed with greatest discriminatory power.

Conclusion: MLVA typing is a faster and more standardized method for studying the genetic relatedness of H. pylori isolates. At preliminary stage it is sufficient to use only 3 VNTR loci for the differentiation of H. pylori strains.

Item Type:	Article
Subjects:	Article Paper Librarian > Biological Science
Depositing User:	Unnamed user with email support@article.paperlibrarian.com
Date Deposited:	04 May 2023 08:10
Last Modified:	06 Feb 2024 04:23
URI:	http://editor.journal7sub.com/id/eprint/814

Actions (login required)

: View Item