Preparation of Macroporous Poly (GMA-DVB) and Its Application to Immobilize β-Galactosidase from Aspergillus Oryzae

The reactive, macroporous glycidyl methacrylate (GMA) and divinyl benzene (DVB) polymer was synthesized with a mixture of cyclohexanol and lauryl alcohol as liquid pore-forming agents and nano-calcium carbonate as solid one via suspension polymerization. The surface structure of the poly (GMA-DVB) beads was characterized with scanning electron microscopy (SEM). Under the optimum conditions, β-galactosidase from Aspergillus Oryzae was bound on the polymer beads obtained above. The activity yield of the immobilized enzyme was 80.35% and the activity of it could reach 540.01 IU/g dry carriers. Meanwhile, the basic properties of the immobilized enzyme, such as optimum temperature, optimum pH, pH stability, thermal stability, operational stability, etc., were investigated.