Determination of Fourier Transform Infrared Spectroscopy (FTIR) Typing an Enterococcus sp.

In the present work, the Fourier Transform Infrared spectroscopy [FTIR] was used to type Enterococcus sp. that was isolated from a hospital in an attempt to develop a standardized procedure for the differentiation and characterization of Enterococci. A rapid and inexpensive method to characterise chemical cell properties and identify the functional groupspresent in the cell wall is Fourier transform infrared spectroscopy (FTIR). Infrared spectroscopy is a well-established technique to identify functional groups in organic molecules based on their vibration modes atdifferent infrared wave numbers. One of the main causes of nosocomial infections, Enterococcus species are challenging to treat due to the emergence of vancomycin resistance. It is crucial for epidemiological research to conduct studies on Enterococcus isolates. Enterococci are frequently typed using both genotypic and phenotypic approaches. In this study, an unique phenotypic method for typing Enterococci is Fourier-transform infrared (FTIR) spectroscopy. FTIR spectroscopy results compared to antibiotic susceptibility testing and PCR amplification of Vancomycin gene results; the analysis showed that, 6 isolates were positive for Van gene (4 of VanA, 1 of VanB and 1 VanA plus VanB). Three of VanA and VanA plus VanB were resistant to all antibiotic tested (Ampicillin, Teicoplamin and Vancomycin) and VanB was found to be sensitive. FTIR spectroscopy (first deriv- atives) divided the isolates into 8 groups. 3 groups of VanA (4 isolates), one of VanB (one isolate), one of VanA plus VanB (one isolate) and the other 13 Enterococcus isolates were divided into 3 clusters. In order to analyze the various molecules and organic chemicals that make up the cell structure, it is possible to ascertain specific physical and chemical properties. The study suggests that certain Enterococci bacterial species produce organic substances that can be used to determine their kind, and it is thought that FTIR has a lot of potential in this area of clinical microbiology.