FASCIOLA LARVAE: ANTHELMINTIC ACTIVITY OF MEDICINAL PLANT Potentilla fulgens AGAINST SPOROCYST, REDIA AND CERCARIA

Fascioliosis caused by trematode Fasciola hepatica and F. gigantica with complex life cycle, and primarily affect in both livestock and human. This disease occurs on all inhabited continents. Humans become infected after ingestion of contaminated food mostly aquatic wild vegetables or water. The life cycle of Fasciola can be interrupted by killing the vector snail or larva (sporocyst, redia and cercaria) in their habitats. In vitro toxicity of dried root powder of Potentilla fulgens, different organic extract and column purified fraction was performed in the Petridis. Infected snail Lymnaea acuminata was dissected in a glass Petridis which containing 10 ml of dechlorinated water at 23oC-25oC. These larvae were kept in dechlorinated tap water where they survive up to 48 h in laboratory condition. Mortality of sporocyst, redia and cercaria were observed after 2 h, 4 h, 6 h and 8 h of treatment. Counting of a larva was done with help of microscope. Per cent mortality of larvae at each concentration for 2 h, 4 h, 6 h and 8 h were used for determination of LC50. In in vitro treatment of different organic extract and column purified fraction of P. fulgens was used. Highest toxicity was observed against sporocyst, redia and cercaria after treatment of column purified fraction (2 h LC50 was 62.42, 59.25 and 45.11 mg/L, respectively). After 8 h LC50 of column purified fraction against sporocyst, redia and cercaria were observed 54.20, 49.37 and 38.13 mg/L, respectively. The present study conclusively shows that medicinal plant P. fulgens have anthelmintic (sporocyst, redia and cercaria) larvicides activities against F. gigantica. It can use in in vivo treatment of infected host snails of Fasciola larvae within the body, which may be useful for control of fascioliasis without killing host snails.