CYTOTOXIC AND ANTIOXIDANT POTENTIAL OF Hybanthus enneaspermus MEDIATED SILVER NANOPARTICLE

Aim: The aim of the study is to synthesis Hybanthus enneaspermus mediated silver nanoparticles and to evaluate the cytotoxic and antioxidant potential.

Introduction: Hybanthus enneaspermus (F. Muell) belongs to the family Violaceae. The plant Hybanthus enneaspermus is reported to have antimicrobial, anticonvulsant, nephroprotective, hepatoprotective, anti-inflammatory, aphrodisiac, aldose reductase inhibitory and free radical scavenging activities. Ag NPs produce reactive oxygen species and free radicals causing apoptosis (cell death) thereby preventing their replication. Many researchers have correlated the plants antioxidant potential with their phenolic constituents. Antioxidants have resistance against oxidative stress by scavenging free radicals, thereby preventing many diseases.

Materials and Methods: Cytotoxic activity: Brine shrimp eggs were obtained from the new aqua laboratory. Filtered artificial seawater was prepared, then shrimp eggs were added into the dark side of the chamber while there was a lamp above the other side to attract the hatched shrimp. Two days were allowed for shrimp to mature, after two days the shrimp Larva was ready. 10 brine shrimps were added accordingly to the silver nanoparticle. It added 5, 10, 15, 20 micrometer and it was left for 24 hrs and observation was made.

Antioxidant activity: 50% methanol, DPPH solution and Hybanthus enneaspermus mediated silver nanoparticles were added in 5 test tubes ranging from 10-50 microliters and kept in a dark place for 10 minutes and the reading was recorded using photometry.

Results and Discussion: In the cytotoxic activity as the concentration of the silver nanoparticle increases the percentage of lethality of brine shrimps also increases. In the antioxidant activity as the concentrations of silver nanoparticles increases the percentage of inhibition also increases.

Conclusion: With the help of the study we can conclude that silver nanoparticles of Hybanthus enneaspermus possess both cytotoxic and antioxidant activity.