The early stage of polyphosphate accumulation in saccharomyces cerevisiae: comparative study by extraction and DAPI staining

Ryazanova, Lubov and Andreeva, Nadeschda and Kulakovskaya, Tatiana and Valiakhmetov, Airat and Yashin, Valerii and Vagabov, Vladimir and Kulaev, Igor (2011) The early stage of polyphosphate accumulation in saccharomyces cerevisiae: comparative study by extraction and DAPI staining. Advances in Bioscience and Biotechnology, 02 (04). pp. 293-297. ISSN 2156-8456

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Abstract

Inorganic polyphosphate (PolyP), a bioactive polymer with multiple functions, plays a key role in biomineralizaion and phosphorus homeostasis in yeasts. After phosphate starvation, the cells of Saccharomyces cerevisiae restored their pool of PolyP during the first 30 min of incubation in the media containing phosphate and carbon sources. The cells of parent strain accumulated PolyP both in glucose and ethanol-containing media. In the medium with glucose, the strain with inactivated PPX1 and PPN1 genes (encoding two major yeast polyphosphatases) accumulated 2-fold more PolyP than the parent strain. The PolyP in the mutant cells had a greater average chain length compared to the parent strain. The strain with inactivated exopolyphosphatase genes РРХ1 and PPN1 was incapable of PolyP synthesis in the medium with ethanol. The in vivo staining of cells with DAPI show that in the cells of parent strain PolyP appeared first in cytoplasm and mitochondria under cultivation in glucose-containing medium and in cytoplasm and vacuoles in the medium with ethanol. In the ΔPPX1ΔPPN1double mutant PolyP accumulated in cytoplasm and vacuoles under cultivation in glucose-contained medium.

Item Type: Article
Subjects: Article Paper Librarian > Biological Science
Depositing User: Unnamed user with email support@article.paperlibrarian.com
Date Deposited: 27 Mar 2023 09:06
Last Modified: 16 Mar 2024 04:54
URI: http://editor.journal7sub.com/id/eprint/442

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